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Recombinant Murine Insulin-like Growth factor-1

Cat:
CGF-043
Product Name:
Recombinant Murine Insulin-like Growth factor-1
GeneID:
16000
Source:
Escherichia coli.
Molecular Weight:
Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
AA Sequence:
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA
Purity:
> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity:
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.
Endotoxin:
Less than 0.1 EU/μg of rMuIGF-1 as determined by LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Usage:
This material is offered by Creative Biomart for research, For research and further manufacturing use only.
For R&D and Further Manufacturing ONLY. Not for Personal Use.

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