Phototoxicity Testing

Cosmetic raw materials are no different from other chemicals in essence, so toxicological research methods for general chemicals or drugs are also applicable to cosmetic raw materials. Phototoxicity is a toxicological effect caused by some chemical substances that absorb ultraviolet light or visible light to generate excited state molecules and oxygen free radicals, the excited state acts on target molecules to produce toxicological effects. Skin will produce light irritation or light sensitivity when exposed to phototoxic substances and exposed to intense light.

Certain types of cosmetics, such as sunscreen products, are mainly characterized by exposing to the sun for a long time. Once the substance is indicated as being phototoxic, the application of such products is likely to have health risks. Therefore, before the product is put on the market, skin phototoxicity testing is required, including the 3T3 neutral red uptake phototoxicity test.

Our Services

The phototoxicity testing service Creative BioMart provides is performed using Balb/c 3T3 mouse fibroblasts based on the OECD guidelines (Organization for Economic Co-operation and Development guidelines for the testing of chemicals: 3T3 NRU phototoxicity test. NO. 432). In order to meet the different needs of customers, we also provide many other cell models, such as HaCaT human keratinocytes.

Information about the phototoxicity testing services we provide:

• Phototoxicity is more common than photoallergy. Most drugs that cause photosensitivity are systemic phototoxicants. Any cosmetics and compounds that absorb UVB, UVA or visible light in the range of 290-700nm and can reach the skin or eyes needs to be tested for potential phototoxicity in accordance with the requirements of regulatory agencies ((FDA Guidance for Industry on Photosafety Testing 2003 and EMEA Note for Guidance on Photosafety Testing 2002).
• Creative BioMart's phototoxicity test is based on a comparison of the cytotoxicity of a test compound when they are tested with and without additional exposure to a non-toxic dose of UVA and visible light. Neutral red (NR) is a cationic dye that easily penetrates cell membranes in a non-ionic diffusion manner and accumulates in lysosomes. Certain chemical substances and external conditions can cause changes in the sensitivity of cell surfaces or lysosomal membranes, leading to irreversible cytotoxic changes such as increased lysosomal fragility, resulting in a decrease in the ability of cells to absorb NR. This method determines whether the chemical substance is phototoxic by measuring the change in the ability of 3T3 fibroblasts to absorb NR after the combined action of chemical substances and ultraviolet radiation.

Creative BioMart is an advanced and professional cosmetic analysis company. We have established comprehensive technology platforms and our well-trained technicians can help customers design, develop, test, and analyze cosmetics, and provide customized one-stop solutions. Our high-quality service and service attitude have made us praised by customers from all over the world.

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For R&D and Further Manufacturing ONLY.