Inhalation Toxicity Testing

In the industrial, pharmaceutical and biotechnology industries, there is an increasing tendency to outsource preclinical research to reliable contract research organizations to manage R&D costs. According to statistics, inhalation toxicology testing services have the highest market share. At present, due to the increase of consumers' awareness of the safety of cosmetics, the use of inhalation toxicology testing services in cosmetics has also greatly increased, which has promoted the growth of inhalation toxicology testing services in the cosmetics market.

This kind of non-regulatory test, also called lung irritation test, can provide information on the damage of the test substance to the lining of the human airway within a certain concentration range. Inhalation toxicity can be specifically defined as the degree to which the test item causes damage (toxicity) to human airway after being exposed to a single substance or mixture for up to 4 hours. It is an important part of product safety assessment and an important part of the occupational safety of manufacturing workers. Various volatile chemical components may cause inhalation toxicity, including cosmetic fragrances, fuels, adhesives, and nanoparticles, etc.

We use IC75 to classify products that are potentially toxic to airway tissue, and rank ingredients or formulations of irritation potential, or compare them against industry benchmarks.

Our Services

We use the 3D human airway tissue model (EpiAirway) to simulate the biochemical and physiological characteristics of the human respiratory epithelial tissue. These cells have been cultured to form a multi-layer highly differentiated model, which consists of a pseudostratified epithelial structure complete with tight junctions as well as microvilli, cilia, and mucin secretion at the apical surface, and very similar to the tracheal/bronchial epithelium in normal humans. EpiAirway grows on the platform of the air-liquid interface, and can accurately simulate the manner of "real life" airway exposure in the test. In some cases, caps are used to prevent excessive evaporation of volatile substances.

We measure cell viability by enzymatically converting the vital dye MTT into blue formazan salt, and then perform quantitative measurement after extracting the salt from the airway tissues. Toxicity is determined by calculating the IC75 value, which is the concentration of the test item required to reduce the viability of the airway model to 75%. If the IC75 value is below the threshold, the test item is used to classify it as toxic.

Creative BioMart is an advanced and professional cosmetic analysis company. We have established comprehensive technology platforms and our well-trained technicians can help customers design, develop, test, and analyze cosmetics, and provide customized one-stop solutions. Our high-quality service and service attitude have made us praised by customers from all over the world.

Welcome to contact us for project quotations and more detailed information.

For R&D and Further Manufacturing ONLY.