Recombinant Murine Fibroblast Growth Factor 16

Cat:

CGF-078

Product Name:

Recombinant Murine Fibroblast Growth Factor 16

Source:

Escherichia coli.

Molecular Weight:

Approximately 23.8 kDa, a single non-glycosylated polypeptide chain containing 207 amino acids.

AA Sequence:

MAEVGGVFAS LDWDLHGFSS SLGNVPLADS PGFLNERLGQ IEGKLQRGSP TDFAHLKGIL RRRQLYCRTG FHLEIFPNGT VHGTRHDHSR FGILEFISLA VGLISIRGVD SGLYLGMNER GELYGSKKLT RECVFREQFE ENWYNTYAST LYKHSDSERQ YYVALNKDGS PREGYRTKRH QKFTHFLPRP VDPSKLPSMS RDLFRYR

Purity:

> 98 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Data not available.

Physical Appearance:

Sterile colorless liquid.

Formulation:

Supplied as a 0.2 μm filtered solution in 20 mM Tris-HCl, pH 9.0, 1 M NaCl, 0.02 % Tween-20, 10 % Glycerol.

Endotoxin:

Less than 0.1 EU/µg of rMuFGF-16 as determined by LAL method.

Reconstitution:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 6 months from date of receipt, -20 to -70 °C as supplied.
- 3 months, -20 to -70 °C under sterile conditions after opening.

Stability & Storage:

This material is offered by Creative Biomart for research, For research and further manufacturing use only.

Usage:

Fibroblast growth factor 16 (FGF-16) belongs to the large FGF family. All FGF family members are heparin-binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-16 was originally identified in rat heart tissue by homology based polymerase chain reaction. Murine FGF-16 cDNA predicts a 207 aa precursor protein with one N-linked glycosylation site. FGF-16 lacks a typical signal peptide, but is efficiently generated by mechanisms other than the classical protein secretion pathway. Among FGF family members, FGF-16 is most similar to FGF-9, sharing 73% aa sequence homology. Murine FGF-16 shares 99.5% and 99% aa sequence identity with the human and rat FGF-16, respectively.