Recombinant Human Neuregulin 1-beta1 EGF Domain, 177-241a.a.

Cat:

CGF-008

Product Name:

GeneID:

3084

Source:

Escherichia coli.

Molecular Weight:

Approximately 7.5 kDa, a single non-glycosylated polypeptide chain containing 65 amino acids.

AA Sequence:

SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE

Purity:

> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 0.5 ng/ml, corresponding to a specific activity of ＞ 2.0 × 106IU/mg.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from a 0.2 μm filtered solution in 1 × PBS, pH 7.4, with 5 % trehalose.

Endotoxin:

Less than 0.1 EU/μg of rHuNRG1-β1, 177-241a.a. as determined by LAL method.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage:

This material is offered by Creative Biomart for research, For research and further manufacturing use only.

For R&D and Further Manufacturing ONLY.

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