Recombinant Human Endocrine Gland-derived Vascular Endothelial Growth Factor

Cat:

CGF-090

Product Name:

GeneID:

84432

Source:

Hs.514793.

Molecular Weight:

Escherichia coli.

AA Sequence:

Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.

Purity:

AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF

Biological Activity:

> 98 % by SDS-PAGE and HPLC analyses.

Physical Appearance:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using bovine EJG cells is less than 2.0 μg/ml, corresponding to a specific activity of > 500 IU/mg.

Formulation:

Sterile Filtered White lyophilized (freeze-dried) powder.

Endotoxin:

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 0.02 % Tween-20.

Reconstitution:

Less than 0.1 EU/µg of rHuEG-VEGF as determined by LAL method.

Stability & Storage:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Usage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

background:

This material is offered by Creative Biomart for research, For research and further manufacturing use only.

For R&D and Further Manufacturing ONLY.

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